iolD [2019-06-24 11:19:39]

formation of 5-deoxy-D-glucuronic acid (3rd reaction)

locus: BSU39730

pI: 5.01

mw: 63.96 kDa

protein length: 637 aa

gene length: 1914 bp

function: myo-inositol catabolism

product: formation of 5-deoxy-D-glucuronic acid (3rd reaction)

essential: no

synonyms: yxdD

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.16|Utilization of inositol]

Gene

Coordinates: 4,079,083 4,080,996

The protein

Catalyzed reaction/ biological activity

• 3,5/4-trihydroxycyclohexa-1,2-dione + H₂O = 5-deoxy-glucuronic acid (according to Swiss-Prot)

Protein family

• TPP enzyme family (according to Swiss-Prot)

Structure

• [PDB|1V5E] (pyruvate oxidase from Aerococcus viridans, 25% identity)

Expression and Regulation

Operons

• genes: [gene|762718A15E5256261D79DF60F9106AF0CE2D60C6|iolA]-[gene|0903CCBE053230998A9123F3269868E952C0F76B|iolB]-[gene|AAE60753464EAA32379521531FDCAF64534D5F40|iolC]-[gene|AA79B6F039EC5B79F4419A5F358AE5AE8292E731|iolD]-[gene|C31AF6C8050D453D9011B0793403791F1851BA69|iolE]-[gene|14813DE6C6DF7AB031BA05AED7D31AE9E8F8498E|iolF]-[gene|93C1DF93CAFC5AE726523A91A3BA7470017FF6DC|iolG]-[gene|FD64E9D00BB03B510C7F7CA3935558A2195532A9|iolH]-[gene|2742FCE60AA87B2BE5A0B5C34F617E10DDE7F126|iolI]-[gene|1EFB5C8F28146D1EA3B615F548D0CF262D2B4DF5|iolJ]
  description: [Pubmed|9226270]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|9226270], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|5F4ABBC8ECAD6F6C10EC39B2B0F0BC73F11522CE|IolR]: repression, [Pubmed|9887260,9226270], in [regulon|5F4ABBC8ECAD6F6C10EC39B2B0F0BC73F11522CE|IolR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|10666464], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • induced by inositol ([protein|search|IolR]) [Pubmed|9887260,9226270]

Biological materials

Mutant

• MGNA-B773 (iolD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1772 NBRP B. subtilis, Japan]
• BKE39730 ([gene|AA79B6F039EC5B79F4419A5F358AE5AE8292E731|iolD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAAACGATCCCACCTTTA, downstream forward: _UP4_CAGTATTAGAGGGGAGGCAC
• BKK39730 ([gene|AA79B6F039EC5B79F4419A5F358AE5AE8292E731|iolD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39730 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACAAACGATCCCACCTTTA, downstream forward: _UP4_CAGTATTAGAGGGGAGGCAC

labs

• [SW|Yasutaro Fujita], University of Fukuyama, Japan
• [[Ken-ichi Yoshida]], Kobe University, Japan

References

• 9226270,18310071,9887260,18310071